Crystal mounting method for the longer wavelength S-SAD phasing

CapLoop2.jpg

Formerly, we named it as the "loopless" crystal mounting method. But now we leave the loop as is after flash freeze the crystal.

References

  1. Kitago, Y., Watanabe, N. and Tanaka, I.:
    Structure determination of a novel protein by sulphur SAD using chromium radiation in combination with a new crystal mounting method,
    Acta Cryst., D61, 1013-1021 (2005).
    http://www.iucr.org/cgi-bin/paper?dz5036

  2. Watanabe, N., Kitago, Y., Tanaka, I., Wang, J., Gu. Y., Zheng, C. and Fan H.:
    Comparison of phasing methods for sulfur-SAD using in-house chromium radiation: case studies for standard proteins and 69-kDa protein,
    Acta Cryst., D61, 1533-1540 (2005).
    http://www.iucr.org/cgi-bin/paper?dz5050

  3. Watanabe, N.:
    From phasing to structure refinement in-house: Cr/Cu dual wavelength system and an loopless free crystal mounting method,
    Acta Cryst., D62, 891-896 (2006).
    http://www.iucr.org/cgi-bin/paper?dz5067

  4. Kitago,Y.,Watanabe, N., and Tanaka, I.:
    Semi-automated protein crystal mounting device for the sulphur SAD method,
    J. Appl. Cryst.., 43, 341-346 (2010).
    http://dx.doi.org/10.1107/S0021889809054272